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Microvascular Health Solutions LLC
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Image Search Results
Journal: European Heart Journal. Imaging Methods and Practice
Article Title: Differential effects of heat-not-burn, electronic, and conventional cigarettes on endothelial glycocalyx
doi: 10.1093/ehjimp/qyad008
Figure Lengend Snippet: Assessment of perfused boundary region of the sublingual arterial micro-vessels, by GlycoCheck software. RBCs, red blood cells; SDF, sideview darkfield imaging; PBR, perfused boundary region. ( A ) SDF camera and GlycoCheck software. GlycoCheck software automatically analyses the recordings and then estimates the PBR for each vascular increment diameter. ( B ) SDF camera utilizes green reflected light-emitting diode (LED) light (540 nm) from haemoglobin molecules to depict the radial displacements of RBCs within the micro-vessels. The camera is inserted under the tongue and captures more than 3000 vascular segments of vessels with diameter ranging from 5 to 25 μ m. ( C ) Example of recording of sublingual micro-vessels by SDF camera. The camera captures more than 3000 vascular segments of vessels. ( D ) Schematic illustration of a micro-vessel, displaying the perfused boundary region of arterial micro-vessel. PBR is calculated by the formula (RBC perfused lumen width—RBC width)/2. ( E ) Each column illustrates micro-vessels’ diameters. PBR within normal range for micro-vessels with diameter 5–25 μ m (mean value of PBR5-25 = 1.95 μ m) indicating intact glycocalyx layer of a subject randomized to use HNBC. ( F ) Each column illustrates micro-vessels’ diameters. Increased PBR (mean value of PBR5-25 = 2.23 μ m) indicating impaired glycocalyx of a subject smoking conventional cigarette, perturbed by red blood cells. The bars on image F are higher than the respective bars on image E .
Article Snippet:
Techniques: Software, Imaging